Fig. 7. Loss of aPKC does not severely alter neurogenesis. (A,B) Coronal section of E16.5 cortex from control (A) and aPKC cKO embryos (B) are stained with antibodies against Ki67 (green) and BrdU (red). BrdU was administrated 24 hours before fixation at E15.5. Arrowheads indicate cell cycle exit cells (BrdU-positive/Ki67-negative). Arrows indicate re-entry cell cycle cells (BrdU-positive/Ki67-negative). (C) The cell cycle exit rate was calculated by dividing the number of BrdU-positive/Ki67-negative cells by the total number of BrdU-positive cells. Results from two independent experiments are shown. Cell cycle exit rate at E15.5 are not significantly affected by the loss of aPKC. (D,E) Coronal section of E16.5 cortex from control (D) and aPKC cKO (E) embryos are stained with antibodies against neurogenin 2 (green) and DAPI (blue). (F) The population of neurogenin 2-positive cells in VZ, SVZ, IZ and SP were quantified and graphed. The rate of neurogenin 2-positive cells is not significantly changed by loss of aPKC. Scale bars: 100 µm in A,B; 25 µm in D,E.