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Figure 6


Fig. 6. NF-AT activity in neuralized animal caps. (A) One-cell stage embryos were co-injected in the animal hemisphere with 20 pg of XBF2 and 0.1 ng or 0.2 ng of CA XNF-ATc3 (CA) or 1 ng of DN XNF-ATc3 (DN) encoding RNAs. (B) RT-PCR analysis was performed with the markers HoxB9, XE10 (EphA2 receptor), Krox20, NCAM and EF1{alpha} (loading control). (C) One-cell stage embryos were injected into the animal hemisphere with 0.2 ng of BMP DN receptor (BMP DNR), 0.2 ng of CA XNF-ATc3- or 1 ng of DN XNF-ATc3. BMP-DNR was also co-injected with either CA XNF-ATc3 or DN XNF-ATc3 at the same concentrations. (D) RT-PCR analysis was performed with the markers HoxB9, HoxD1, XAG-1 and EF1{alpha} (loading control).