Fig. 5. Molecular analysis of vps-35. (A) Schematic of vps-35 genomic region on chromosome II (LGII). Arrows depict hypothetical gene transcripts and direction of transcription (www.wormbase.org). Cosmid clone F59G1 (2/2 lines) and a 5.6 kb PstI-MscI genomic fragment (3/3 lines) (shown) rescued vps-35 mutants. Based on rescue and expression studies, vps-35 is likely to be the first gene in a multigene operon rather than tsp-18, as indicated on WormBase Release WS150. (B) vps-35 gene structure is based on cDNA and genomic sequences. vps-35 transcript is trans-spliced to SL1. Our results confirm essential features of vps-35 (F59G1.3) (WormBase Release WS150), except that the initiation ATG is at position 5907784 rather than 5907868. The deletion in vps-35(zd163) is marked. Black boxes indicate exons and the white box depicts the 3' UTR.