Fig. 3. tsr PCP defects in adult epithelia.
tsr
96 flies rescued by
tsr139/tsrV27Q or P[WHTG] have PCP defects in
several epithelia. (A-C) The wing (anterior is upwards and distal is
rightwards). (A) Wild-type wings always have a uniform and distally
pointing hair orientation; (B)
tsr139/tsrV27Q wing hairs have a non-distal
orientation; and (C) a P[WHTG] wing has hairs oriented in swirls or
non-distally (not shown). (D) The eye. A thin section through a
heat-shocked P[WHTG] eye shows a field of ommatidia that have randomly adopted
polarities. (E) The abdomen. A P[WHTG] cuticle shows a random
orientation of fine hairs. A region of hairs with wild-type orientation that
points posteriorly is shaded. (F-H) The leg. A wild-type leg (F) shows
normal segmentation and bristle pattern. Blue, tarsal segment 3; yellow, the
correct joint position and polarity. P[WHTG] legs (G,H) have aberrant tarsal
segments. Blue, the equivalent of tarsal segment 3 (from tibia); yellow,
aberrant joints with duplications. At higher magnification (I), some
P[WHTG] leg bristles show reversed polarity as do the `bract-socket vectors'
(Held et al., 1986). Bracts
(purple) are fine hair-like structures at the base of each bristle: two
bracts, one above the other, have the correct polarity: growing from the
proximal side of the socket and point distally (to the right). The third bract
has reversed polarity, growing from the distal side of the socket and points
proximally. (J) The notum. A
tsr139/tsrV27Q cuticle
shows some of the fine hairs have lost the proper posterior-pointing
orientation. Occasionally multiple hairs are observed (arrow).