Fig. 5. Fz-GFP and Fmi are mislocalized in tsr mutant wings. Shown are: Fz-GFP (A,D,F); Fmi (B,E,G); and merged images of Fz-GFP in green and phalloidin-stained F-actin in red (C,F,I). (A-C) A wild-type wing, prior to prehair emergence aged greater than 48 hours APF at 18°C. Fz-GFP and Fmi are asymmetrically localized in a zigzag pattern at the PD boundary. The merged image shows Fz-GFP and Fmi colocalization. (D-F) A moderately affected tsr¹³⁹/tsr^V27Q wing of similar age shows an interrupted asymmetrical distribution of Fz-GFP. Fz-GFP is frequently missing from cell vertices and cell sides (asterisk). Similarly, Fmi shows an interrupted asymmetrical distribution. The merged image shows the extent of colocalization. (G-I) A severely affected tsr¹³⁹/tsr^V27Q wing shows that the recruitment of Fz-GFP to cell boundaries is largely lost, whereas Fmi is still enriched at cell boundaries where it shows an uneven and interrupted distribution. The merged image shows the zigzag pattern was interrupted.