Fig. 6. Prehairs do not initiate from the correct location in tsr mutant wings. Fz-GFP (A,E,I); Fmi (B,F,J); F-actin (C,G,K); and merged images of Fz-GFP in green and phalloidin-stained F-actin in red (D,H,L). (A-D) A wild-type wing during prehair initiation aged 64 hours APF at 18°C. Fz-GFP and Fmi show the characteristic asymmetrical distribution at the PD boundaries. F-actin accumulations show a single prehair centered at the distal-most vertex of each cell (C). The merged image shows the overlay of Fz-GFP (green) and F-actin (red) localization. (E-H) A moderately affected tsr¹³⁹/tsr^V27Q wing of a similar age shows a Fz-GFP distribution that was interrupted at the distal cell boundaries. (E) Fz-GFP accumulated unevenly and was missing from some cell boundaries. (F) Similarly, Fmi shows an uneven distribution at PD cell boundaries. (G) The F-actin accumulation shows prehairs were not centered through the distal vertices; few were extended. (H) The merged image. (I-L) tsr¹³⁹/tsr^V27Q, a severely affected wing shows that Fz-GFP is not enriched at PD boundaries and the Fmi distribution at PD boundaries is uneven showing puncti of strong staining alternating with gaps in the staining pattern. Phalloidin-stained F-actin (K) shows prehairs abnormally formed near cell centers (arrowhead). The merged image shows the extent of Fz-GFP delocalization.