Fig. 8. Overexpression of a constitutively active Limk results in wing PCP defects. The activated Limk protein was expressed using an ap-Gal4 promoter construct that only expresses on the dorsal wing blade. Transgenic flies grown at 23°C survive and show defects with wing hair polarity similar to those seen in fz class wings (A), or have wing hairs that show a more chaotic wing hair orientation pattern (B). (C) A pupal wing, treated with phalloidin, on which the ventral surface hairs are oriented correctly (left panel), but hairs on the dorsal surface show the PCP defect (right panel). (D) A pupal wing with prehairs emerging correctly from the distal-most vertices on the ventral blade (left panel), while the dorsal blade (right panel) shows a phenotype similar to the tsr¹³⁹/tsr^V27Q mutant (E).