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Figure 3


Fig. 3. Increased apoptosis following inactivation of Shh signaling at E9.0. TUNEL assay on control, Smo-En1 cko and Smo-Nes cko mutants. (A-D) Apoptotic cells (green) are increased in E9.5 Smo-En1 cko mutants, particularly in the anterior mes and around the isthmus (B, arrowheads) but not in the ventral midline (D, red arrowhead). (E,F) A few apoptotic cells are found in control and E12.5 Smo-Nes cko mutant embryos (arrowheads), but cell death is not increased in the mutants. Hoechst counterstaining is in blue. (G) Shown areas are indicated in the schematic. V, ventral; D, dorsal. Scale bars: 25 µm.