Fig. 4. Laminar organization is grossly normal in Gli2-En1 cko cerebella. The internal granule layer (IGL) containing differentiated granule cells marked by NeuN is extremely sparse in mutants (D) compared with wild type (A). The PCs, marked by calbindin, are multilayered with less elaborate dendritic arborization in mutants (E), in contrast to the monolayer and complex arborization in wild type (B). The Bergmann glia marked by BLBP in green, are interspersed with the PCs (red); however, the glial fibers appear disorganized in mutants (F) compared with wild types (C).