Fig. 2. Eph and Ephrin are targeted to distinct neuronal compartments. (A-C) View of approximately five segments of the ventral nerve cord of an unfixed dissected stage 16 embryo double-labeled with the panaxonal BP102 antibody (A) and Ephrin-Fc (B). Ephrin-Fc binds to axons in a pattern that essentially overlaps that of BP102. (D,E) Homozygous Eph^x652 embryo double-labeled with the panaxonal anti-HRP antibody (D) and Ephrin-Fc (E). Homozygous Eph^x652 embryos lack all Ephrin-Fc binding, confirming the specificity of Ephrin-Fc binding and that Eph^x652 is null for Eph function. (F) Ephrin-Fc binding is unaffected in homozygous onecut^lx122 embryos. (G-I) A scrt-Gal4/+;UAS-Ephrin:myc/+ embryo double labeled with the panaxonal anti-HRP antibody (F) and anti-myc antibody(G). scrt-Gal4 drives expression of Ephrin:myc in all neurons where it is targeted to cell bodies. Anterior is upwards.