Fig. 4. Eph^x652 mutant embryos lack obvious axon guidance defects in the embryonic CNS. View of the ventral nerve cord of stage 15-16 embryos in wild type (A,C,E) and Eph^x652 mutants (B,D,F). (A,B) Embryos labeled with BP102 to visualize all axons. No obvious defects in axon guidance are detected in Eph^x652 mutant embryos (B). (C,D) Staining with anti-Fas2 antibody, which labels five distinct axon bundles running anteroposteriorly on each side of the midline, three of which are in the plane of focus, is normal in Eph^x652 mutants (D). (E,F) Embryos double-labeled for Fas2 (red) and for ß-galactosidase (green) driven by the expression of apC-tau-lacZ to visualize axonal projections of Ap neurons. Ap neurons extend axons (arrows) normally along the medial Fas2 bundle in Eph^x652 mutants (F). Genotypes: (E) apC-tau-lacZ/+; Eph^x652/+; (F) apC-tau-lacZ/+;Eph^x652/Eph^x652. Anterior is upwards for all panels.