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Figure 1


Fig. 1. Transcriptional regulation of the Dlx3 promoter by p63. (A) Fold-induction increase, compared with wild type, of Dlx3-117/+60 cotransfected with vectors expressing the different p63 isoforms. (B) Sequence of the mouse Dlx3-promoter region containing the two overlapping p63-binding sites (p63 site1 and p63 site2; underlined) and CCAAT box (italics). The mutated Dlx3 sequences are shown in gray. (C-D) Dlx3-117/+60, Dlx3p63M1 and Dlx3p63M2 constructs were cotransfected with vectors expressing the TAp63 (C) and {Delta}Np63 (D) isoforms, with activity shown relative to the basic activity of Dlx3-117/+60. Transient transfection experiments were performed using mouse keratinocytes (C-D) and Saos-2 cells (E). Basal activity of the reporter was set to 1. Each histogram bar represents the mean of three independent transfection duplicates. Standard deviations are indicated. Dlx3 p63M1, mutated at p63-binding-site 1; Dlx3p63M2, mutated at p63-binding-site 2.