Fig. 3. p63 and Dlx3 colocalize in the embryonic ectoderm, and Dlx3 expression is downregulated in p63-KO embryos. (A) Histochemistry with anti-distal-less (pan-anti-Dlx; red) and p63 (green) antibodies on the dorsal forelimb ectoderm of E10.5 wild-type embryos (merge of Dlx and p63 expression is yellow). DAPI staining is also shown (blue). Arrows indicate the dlx-p63 double-positive nucleus. (B) Relative mRNA abundance, determined by real-time PCR, for Dlx3, TAp63 and Np63 in the anterior limb (AL) and posterior limb (PL) of E10.5, E11.5 and E12.5 wild-type embryos. The relative abundance is expressed as fold-induction relative to the value at E10.5 (set at 1). (C) Histochemistry with anti-p63 and anti-Dlx on E10.5 wild-type and p63-KO limb-bud ectoderm. (D) Whole-mount in situ hybridization on wild-type and p63-KO E10.5 embryos with a Dlx3 antisense probe. Arrows indicate limb buds. M, mesoderm; E, ectoderm. Scale bars: 10 µM in A and 40 µM in C. wt, wild type.