Fig. 3. Oogenesis arrest in egg mutants. (A) Ovaries labeled for the germ-cell-specific protein Vasa (green) and a DNA dye (red). Regions of the wild-type germarium are indicated in the top panel. egg¹⁴⁷³ ovaries consist of germaria that fail to bud off normal egg chambers. These germaria often exhibit no recognizable regions and are filled with germ cells and germline cysts that do not form apparent egg chambers (middle panels). In some cases an incompletely budded single egg chamber lies at the posterior end of the mutant germarium (white arrow; bottom panel). (B) Ovaries labeled with an antibody to Fas III (Fas3, green) and a DNA dye (red). Incomplete encapsulation of germline cysts occurs in egg¹⁴⁷³ germaria, and the prefollicular (arrowhead) and follicle cells (arrow) appear reduced in number. WT, wild type.