Fig. 5. egg is required for trimethylation but not dimethylation of H3K9 in germ and somatic cells during oogenesis. Left panels, methylated H3K9 alone; right panels, merged images of H3K9me3 or H3K9me2 (green) and Vasa (red). (A) H3K9me3 is absent or severely reduced in both the germ and somatic cell nuclei of egg¹⁴⁷³ germaria. (B) High levels of H3K9me2 are present in both germ and somatic cell nuclei of egg¹⁴⁷³ germaria.