Fig. 4. Increased glycogen content in G6-Nkx DH lung epithelium. G6-Nkx DH lungs have increased glycogen deposits, as measured by transmission electron microscopy (A-F), and increased PAS staining (G,H, red staining and arrowheads). Lamellar bodies were observed in wild-type and single heterozygous lung epithelium (B-D, arrowheads) but were absent or only rarely found in G6-Nkx2.1 DH lung epithelium (E,F). Scale bars: 10 µm in A,E; 2 µm IN B-D,F; and 50 µm in G,H.