Fig. 6. Genes involved in phospholipid production and processing are downregulated in the lungs of G6-Nkx DH embryos. Q-PCR was performed on E18.5 lung tissue from wild-type, Gata6^+/-, Nkx2.1^+/- and G6-Nkx DH embryos using the oligonucleotides listed in Table 3. (A) Scd1, Dpp4, RBP-L and napsin are all downregulated to a small extent in Nkx2.1^+/-, but not Gata6^+/-, lungs. However, all four genes are severely downregulated in G6-Nkx DH lungs. (B) Comparison of changes in gene expression of Scd1, Dpp4, RBP-L and napsin from microarray and Q-PCR experiments. (C) In situ hybridization performed on wild-type and G6-Nkx DH lungs using riboprobes for RBP-L, Dpp4, napsin and Scd1 shows reduced expression of all four genes in G6-Nkx DH lungs at E18.5. Scale bars: 500 µm in C.