Fig. 9. Activation status of Akt, p70S6 kinase, mTOR and MAPK in oocytes of WT and Tg mice. Oocytes were isolated from 15- to 17-day-old WT or Tg mouse ovaries and lysed. Samples containing 50 µg of protein were analyzed by western blot. Levels of activated Akt (A), activated p70S6 kinase (B), activated mTOR (C), total mTOR (D), activated MAPK (E), total MAPK (F), and ß-actin (G) as an internal loading control, are shown. The experiments were repeated three times; for each experiment n=15-20 for each genotype. Representative results are shown.