Fig. 7. ES cells derived from Ncad-GFP embryos can form epithelial structures in teratomas. (A,A') Histological stainings with hematoxylin and eosin (H&E) on paraffin sections of heterozygous (A) and homozygous (A') Ncad-GFP k.i. teratomas. Multiple epithelial-like structures are seen on both sections. (B,B') Anti-GFP immunolabeling reveals expression of N-cad-GFP in epithelial cells. (C,C') On sections consecutive to B and B', expression of E-cad protein is detected in heterozygous teratomas (C), and is absent in homozygous Ncad-GFP teratomas (C'). (D,D') Epithelia are formed in heterozygous (D) and homozygous Ncad-GFP teratomas (D') and are positive for cytokeratin 8 immunolabeling. Scale bar: 100 µm.