Fig. 2. Distinct binding specificity of different class 3 semaphorins and VEGF164 for blood vessels and nerves. AP-tagged VEGF164 (A,G), SEMA3A (B,H), SEMA3C (C,I), SEMA3E (D,J), SEMA3F (E,K) and AP alone as a negative control (F,L), were reacted with 11.25 dpc mouse hindbrain tissue to examine their binding preference for growing vessels in the subventricular zone (A-F) or axon tracts on the pial brain surface (G-L; arrowheads); binding to vessels entering the brain from the perineural vascular plexus is indicated with arrows. Each panel is 0.25 mm².