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Figure 3


Fig. 3. nab limits distal expression of wg in the inner ring. (A) nabSH143 FRT80 clones in the Drosophila wing pouch labeled by the loss of GFP (green) and stained with Wg antibody (red). wg is misexpressed in these clones (arrows). (B,C) nubGal4/UASGFP (B) and nubGal4/UASGFP; UASnab/+ (C) wing discs stained with Wg antibody (red). nab misexpression represses activation of wg expression by the IR enhancer. Arrows, IR; arrowheads, OR. (D,D') Clones of nab misexpression (green): y w hsFLP122; Act5C>y+>Gal4 UASGFP/+; UASnab/+. wg expression (red) in the IR is cell-autonomously repressed. D' shows the individual channels for the selected areas in D.