Fig. 5. COBRA of wild-type and myr-Akt-Mer GS cells (left) and offspring derived from myr-Akt-Mer GS cells (right). GS cells on laminin were cultured for 6 days in the indicated conditions. The PCR products of each DMR region were digested with the indicated restriction enzymes with recognition sequences containing CpG in the original unconverted DNA. Black and white arrows indicate the sizes of the unmethylated and methylated DNA fragments, respectively. Levels of percentage methylation, as estimated by the intensity of each band, are indicated below the gels. C, cleaved; U, uncleaved.