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Figure 5


Fig. 5. Mesenchymal and ectodermal Pygo2 cooperate in mouse lens development. (A,C,G,I) Whole-mount embryos unstained (G,I) or visualized for GFP (A,C). (B,D-F,H,J,K-N) Unlabeled, DIC-illuminated cryosections (H,J) and cryosections labeled for nuclei (B,E,F, blue), GFP (B,D, green), F-actin (D, red), Pygo2 (E,F, red), Pax6 (K-N, green) and Ap2{alpha} (K-N, red). In E,F, the dashed line encloses approximately equivalent regions of mesenchyme and surface ectoderm. In M,N are shown the green (Pax6) and red (Ap2{alpha}) channel intensities for a line interval passing through the nuclei of the lens placode. ov, optic vesicle; 1ba, first branchial arch; otv, otic vesicle; ple, presumptive lens ectoderm; om, ocular mesenchyme; lp, lens placode; pr, presumptive retina. A gray line between panels indicates that different channels of the same image are displayed. (O) Box plot showing the ratio of lens to optic cup diameter in E12.5 embryos of the indicated genotypes. Mutant values (green and red bars) were significantly different from the wild-type control (yellow bar); * within the box, P≤0.05>0.01 (Wnt1-cre); ** within the box, P≤0.01≥0 (Le-cre, Le-cre; Wnt1-cre double, Ap2{alpha}-cre, germline null). Conditional mutant values (green bars) were significantly different (**, P≤0.01≥0) from the germline null (red bar). Wnt1-cre and Le-cre conditional mutants were also significantly different (**, P≤0.01≥0) from the Ap2{alpha}-cre conditional mutant. (P) Box plot showing the ratio of average Pax6:Ap2{alpha} labeling intensity across the lens placode (as in Fig. 4M,N and Fig. 5M,N) for the genotypes indicated. Mutant values (green and red bars) were significantly different from the wild-type control (yellow bar); ** within the box, P≤0.001≥0). Conditional mutant values (green bars) were significantly different (** within the box, P≤0.001≥0) from the germline null (red bar). Wnt1-cre conditional mutants were also significantly different (** within the box, P≤0.001≥0) from the Ap2{alpha}-cre conditional mutants.