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Figure 7


Fig. 7. Absence of ectopic GFAP expression in oligodendroglial precursors of the Olig2-ablated cortex. (A-B'') The Olig2 (CkoG) mutant mouse cortex at P14 was immunostained with GFAP and OPC markers NG2 (A-A'') and PDGF{alpha}R (B-B''). Regions of the superficial layers of Olig2- ablated cortices are shown. Expression of GFAP is distinct from that of OPC markers. (C-F) Coronal sections of control (CtrlC) and Olig2Cko;CNPCre (CkoC) mutant cortices at P14 were subjected to in situ hybridization labeling for Mbp (C,D) and Gfap (E,F). Arrows indicate the cortical gray matter. (G,H) The corpus callosum of control (CtrlC) and Olig2 (CkoC) mutant cortices at P14 were immunostained with antibodies against GFAP (green) and GS (red).