Fig. 5. Induced AP1 activity can transcriptionally repress SVP and SOC1. Transcript levels were determined by quantitative real-time PCR analyses of three independently collected replicates. Results were normalized against the expression of TUB2, then against the value of the first set of samples. Error bars indicate SD. (A,B) Timecourse expression of SVP (A) and SOC1 (B) in inflorescence apices of ap1-1 35S:AP1-GR plants mock-treated (Mock) or treated with 10 µM dexamethasone (Dex). (C) Expression of SVP and SOC1 in inflorescence apices of ap1-1 35S:AP1-GR, which were mock-treated (M), treated with 10 µM dexamethasone (D), with 10 µM cycloheximide (C) and with 10 µM cycloheximide plus dexamethasone (CD). Expression analyses were performed after 4 hours of treatment. (D) Expression of AGL24, SVP and SOC1 in inflorescence apices of wild-type and ap1-1 plants. Gene expression in wild-type Arabidopsis plants was set to one.