Fig. 3. Deficit of KA' interneurons is independent of apoptosis in mib^-/- zebrafish embryos. Dorsal views of the posterior neural plate at 14 hpf (A,C) and lateral views of spinal cord at 25 hpf (B,D) in Tg(olig2:egfp) (A,B) and mib^-/-;Tg(olig2:egfp) embryos (C,D). Green, blue and red channels mark EGFP⁺ cells, Isl⁺ neurons and TUNEL⁺ cells, respectively. Neither control nor mib^-/- embryos had EGFP⁺ TUNEL⁺ cells at 14 hpf or 25 hpf (A-D). TUNEL⁺ Rohon-Beard neurons (arrowheads) were detected in control and mib^-/- embryos (B,D) at 25 hpf. Scale bar: 25 µm.