Fig. 6. Na,K-ATPase 2 and Ncx4a in DFCs/KV are required for LR patterning. (A) Morpholino knockdown of genes encoding Na,K-ATPase 2 or Ncx4a in DFCs induces LR patterning defects in zebrafish. Bar chart shows the percentage of embryos injected with 2-MO or Ncx4a-MO developing cardiac looping defects. (B,C) Fluorescent signals of the 2-MO accumulated in DFCs in embryos injected with the MO at the 128-cell stage (B), but were largely excluded from DFCs when the injection was done at the dome stage (C). Arrows point to DFCs. (D,E) The expression pattern of ntl in 2 morphants (E) is similar to that observed in CTL-MO-injected embryos (D). (F,G) The morphology of KV (arrowheads) appears normal in control (F) and 2-MO-injected (G) embryos. (H,I) Anti-acetylated tubulin staining shows that cilia are present in KV of control (H) and 2-MO-injected (I) embryos.