Fig. 1. Injected grk RNA moves from the nurse cells into the oocyte and localizes specifically, in the same way as the endogenous transcript. (A,C,E,G) grk RNA (A,C,E) and bcd RNA (G) localization at 15 minutes after injection; (A',C',E',G') at 60 minutes after injection. (B,D,F,H) In situ hybridization showing endogenous grk mRNA (B,D,F) and bcd mRNA (H) in equivalent-stage egg chambers (RNA in red, DNA in cyan). (A) Stage 7 (also see Movie 1 in the supplementary material); (C) young stage 8; (E,G) stage 9 (also see Movie 2 in the supplementary material) egg chambers. grk and bcd RNA injected into nurse cells is transported into the oocyte and localizes in the same way as the endogenous mRNAs. (A',E',G) Arrows indicate sites of apparent ring canal accumulation. (I) lacZ RNA (pink) was injected into the nurse cell, as shown by the outline of the egg chamber (grey). (I',I'') At 15 (I') and 60 (I'') minutes after injection. Injected lacZ RNA accumulates in the nurse cells, with little movement into the oocyte and no localization. In all panels, anterior is to the left and dorsal is to the top. Scale bars: 40 µm.