Fig. 3. grk RNA particles move in directed paths towards ring canals. (A,B) Injected and subsequently fixed egg chambers show that grk RNA (A) but not lacZ RNA (B) accumulates at the ring canal (arrows). (B) Arrowhead points to a ring canal itself. (C) Frames from a time-lapse movie of a nurse cell injected with grk RNA (red) in an Actin-GFP (green) egg chamber (also see Movie 4 in the supplementary material). Panels correspond to successive time points, at 5 second intervals. Arrows indicate two grk RNA particles moving on the same path towards a ring canal that is connected to the oocyte. (D) grk RNA (red) accumulation at the ring canal at approximately 3 minutes after injection. (E) grk RNA particle (red) moving through a ring canal shown as a trail, successive time-points (at 5 second intervals) being superimposed on each other. Notice the difference in distances between RNA particles moving through the ring canal (E) and those moving towards ring canals (C). At the bottom of the figure is a table showing the corresponding grk RNA particle statistics. In all panels, the oocyte is to the right. (C-E) Red is grk RNA; green is Actin-GFP. Scale bar: 5 µm.