Fig. 5. Dynein is required for the efficient transport of grk RNA into the oocyte and for its localization. (A-D) Wild type (A,B; wt) and dhc mutant (C,D; dhc) stage-8 egg chambers injected with grk RNA (pink) in the nurse cells. Notice the decrease in grk RNA transport and localization in the oocyte in dhc mutants (compare B' with D') (also see Movie 5 in the supplementary material). (A-D) Grey is brightfield illumination showing the outline of the nuclei and oocyte. Arrowheads indicate the position of the oocyte nucleus. (A',B',C',D') Injected grk RNA only. (E-L) grk (E-H) and osk (I-L) mRNA in situ hybridization in wild-type (E,F and I,J; wt) and dhc mutant (G,H and K,L; dhc) egg chambers. (E,G,I,K) Stage 3-4 egg chambers; (F,H) stage 6 egg chambers; (J,L) stage 11A egg chambers. Notice the reduction in grk and osk mRNA signal and localization in the oocyte of dhc mutants (arrowheads; compare E' with G' and F' with H' for grk mRNA, and I' with K' for osk mRNA). (L) In late-stage egg chambers, cytoplasmic foci of osk mRNA on the periphery of dhc mutant egg chambers is observed (arrows). (E-L) Cyan is DNA; red is mRNA; black and white panels show mRNA only. In all panels, anterior is to the left and dorsal is to the top.