Fig. 1. EBs containing Gli1^FLAG exhibit an amplified response to Hh stimulation. (A-P) Immunostaining of EBs to detect neural progenitor types in mouse. While both control (A-H) and Gli1^FLAG samples (I-P) respond to Hh signaling by activating Nkx6.1 expression (F,N), only the latter samples contain Nkx2.2⁺ and FoxA2⁺ cells on Hh-Ag treatment (O,P). (Q) Distinct neural progenitors demarcated by expression of progenitor-type-specific markers were counted and represented as a percentage of all DAPI-positive cells in three independent EBs. The error bars represent the standard deviation. Scale bar: 100 µm.