Fig. 5. Decreased expression of N-myc and Hop in Foxp2^-/-;Foxp1^+/- compound mutant mice. In situ hybridization was performed on wild-type (A,C,E,G,I,K,M,O) and Foxp2^-/-;Foxp1^+/- compound mutants (B,D,F,H,J,L,N,P) at E16.5 to determine the lung epithelial expression of genes encoding SP-C (A,B), Nkx2.1 (C,D), Shh (E,F), SP-B (G,H), Foxa2 (I,J), Gata6 (K,L), N-myc (M,N), and Hop (O,P). (Q) Expression of N-myc and Hop was significantly decreased in Foxp2^-/-;Foxp1^+/- compound mutants, whereas expression of Nkx2.1 was not significantly affected as measured by Q-PCR. Scale bars: 100 µm in A,B; 500 µm in C-P.