Fig. 5. Morphogenetic defects in the lulu neural plate. (A-D) Transverse sections of E8.5 wild-type (WT; A,C) and lulu mutant (B,D) embryos. (A,B) Sox2 antibody (green) labels the pseudo-stratified neural plate (dorsal) and the columnar gut endoderm (ventral). E-cadherin (red) labels the gut endoderm and the cuboidal surface ectoderm lateral to the neural plate. The neural plate in lulu mutants appears thickened and broader than in wild type, and the foregut fails to close. (C,D) Phospho-histone H3 (green)-labeled mitotic nuclei are apical in wild type (C); some mitotic nuclei are not apical in lulu (arrows, D). Phalloidin (red) shows cell shape. (E,F) 3D reconstructions of DAPI-stained nuclei (representatives false-colored) in wild-type (E) and lulu (F) anterior neural plates; the apical surface (asterisks) and apical-basal axis (double-headed arrows) are indicated. Wild-type nuclei (E) are ellipsoid and align with the apical-basal axis (cyan nucleus). lulu nuclei (F) vary in shape (spherical nucleus, yellow) and appear to align randomly (magenta nucleus). Dorsal is up in all panels. Scale bars: 20 µm in A-D; 5 µm in E,F.