Fig. 7. Cytoskeletal alterations in the lulu neural plate. (A-H) Transverse sections of E8.5 wild-type (WT; A,C,E,G) and lulu (B,D,F,H) anterior neural plates; boxes in A,B show the equivalent positions of the high-magnification images in C,E,G and D,F,H, respectively. (A-D) Phalloidin reveals F-actin localization and tissue shape; ectopic concentrations of F-actin appear away from the apical surface of the lulu neuroepithelium (arrowheads, D). (E,F) Myosin IIB is concentrated at ectopic sites in the lulu neural plate (arrowheads, F). (G,H) Anti-phospho-ERM antibody recognizes activated (actin-binding) ERM proteins; lulu neural plates show ectopic phospho-ERM staining away from the apical surface. Scale bars: 25 µm in A,B; 5 µm in C-F; 10 µm in G,H.