Fig. 4. Salivary gland positioning is disrupted in dnt and Drl-2 mutant embryos. (A-D) Lateral views of stage 15-16 Drosophila embryos stained with FKH and CRB. (E) Graphical representation of phenotypic penetrance in embryos scored for salivary gland migration defects at stages 14-16. (A) Wild-type control. (B) dnt^e00722 mutant embryos have salivary glands that curve ventrally at the tip of the salivary gland. (C) Similarly, Drl-2-null embryos also show a derailed-like phenotype. (D,E) Salivary gland curving is also seen in drl^R343 Drl-2^E124 double mutants, but the penetrance of the phenotype is not enhanced compared to either of the single mutants.