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Figure 2


Fig. 2. Ena localization. Wild-type embryos, anterior left, antigens indicated. (A, inset) Cellularization, surface view. (A) Gastrulation, ventral view. (B-D) Stages 10 and 12. Arrows, amnioserosa; blue arrowheads, tricellular junctions. White arrowhead in D', leading edge. (E,H) Onset of dorsal closure, (F,G,I) mid-late dorsal closure: arrowheads, Ena at leading-edge AJs; blue arrowheads, tricellular junctions. Arrows in F,G, segmental groove cells; arrows in H,I, dorsal-fold. (J,K) Live embryos. GFP-Ena at filopodial tips (J, arrowhead) and leading-edge AJs (K, arrowhead). (L,L') FP4mito (paired-Gal4). FP4mito recruits Ena from cytoplasm and AJs to punctate structures (blue arrows). Adjacent wild-type cells retain Ena at amnioserosal cell cortex (white arrow) and leading edge AJs (arrowhead). (M) AP4mito (engrailed-Gal4) does not recruit Ena. (N,O) FP4mito (green, paired-Gal4). (N',O') Expression boundaries (arrowheads). Unaltered cortical actin and actin/myosin cable (N), DE-cad (O). (P) UAS-Ena (engrailed-Gal4) elevates Ena levels (P'') without altering cable or cortical actin (P'). Scale bars: 20 µm.