Fig. 9. Altering Ena levels or localization alters membrane protrusions. (A-E,G) Movie stills showing embryos expressing GFP-actin alone (A) or with FP4mito (B,C), UAS-Ena (Ena overexpression; D,G) or FP4CAAX (E) using engrailed-Gal4. Asterisk, engrailed stripe in high-magnification series. Time, minutes:seconds. (A) See Movie 5 in the supplementary material. Wild-type. Arrow, lifecycle of lamellipodial-based protrusion. (B) See Movie 5 in the supplementary material. FP4mito. Lamellipodia and rare, short filopodia (arrow, 4:00). (C) FP4mito. Arrow, lamellipodia extended for several minutes; arrowhead, second lamellipodium emerging as first retracts. (D) See Movie 7 in the supplementary material. Ena overexpression. Arrows, fusion of microspikes to form filopodia. (E) FP4CAAX. Longer filopodia. (F) Box and whisker plot, maximum filopodial length in leading-edge cells, genotypes indicated. Box, 25th-75th quartile; line across middle, median; diamond, mean±s.d.; broken lines, 10th and 90th percentiles; dots, outliers. P values, Student's t-test. (G) Ena overexpression induces filopodia on lateral cells. Scale bars: 5 µm.