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Figure 1


Fig. 1. Induction of trichome initiation by cytokinins in wild-type Arabidopsis plants and GIS clade mutants. (A) Trichome initiation on sepals (top panel) and first (bottom panel) and third (middle panel) cauline leaves of wild-type, gis, zfp8, gis2 and gis gis2 ZFP8-RNAi line 2 (termed gis ZFP8-R gis2) plants that were treated with increasing concentrations of 6-benzylaminopurine. Values represent averages and standard error for 20 plants. (B) Expression of ZFP8 and GIS2 in loss-of-function mutants and RNAi lines; left panel: RT-PCR analysis of GIS2 expression in gis2 and gis ZFP8-R gis2 mutants; right panel: real-time PCR analysis of ZFP8 expression in wild-type (1), zfp8 (2), gis ZFP8-R1 (3), gis ZFP8-R2 (4), gis2 ZFP8-R1 (5), gis2 ZFP8-R2 (6), gis ZFP8-R1 gis2 (7) and gis ZFP8-R2 gis2 (8). Values are ratios to wild type of normalized transcript levels. Sepal trichome values represent the total number of trichomes for 12 flowers. WT, wild type.