Fig. 3. Two HRE sites in the dpp 3'UTR mediate the binding and repression of HOW. (A) Schematic representation of the two HRE sites in the 3'UTR of dpp. These sites were mutated as described. (^*) Wild-type sequences; () mutated sequence. (B) Western blot analysis for HA-tagged HOW or HOW^m (HOW-HA) following precipitation with the wild-type (WT) or mutated dpp 3'UTR. (C) Western blot analysis with anti-GFP, anti-actin and anti-TAP of S-2R+ cells transfected with GFP-dpp3'UTR containing wild-type or mutated HRE sites, together with or in the absence of HOW(L). The bar graph represents the results of three independent transfection experiments that were normalized to the actin levels of each sample.