Fig. 6. Presence of ectopic cartilage masses near Kif3a-deficient synchondroses. Sections of P7 and P15 control (A-C) and mutant (D-F) synchondroses were stained with hematoxylin and Eosin (H&E) or processed for in situ hybridization analysis of collagen II expression. Ectopic cartilaginous masses forming in mutant specimens (arrows in D-F) are recognizable by their typical histology and expression of collagen II. Such a phenomenon is never observed in control specimens, in which the chondro-perichondrial boundary is clear and unviolated (A-C). Scale bar: 75 µm in D for A-F.