Fig. 2. Mutations in the Drosophila pins gene affect aster formation without altering the density of spindle MTs. Cells were stained for tubulin (green), DNA (blue) and Deadpan (not shown, but see Fig. 3A below) to identify NBs. (A) Prometaphase, (B) metaphase, (C) anaphase and (D) telophase from pins^P62 mutant brains. (E) Frequencies of NBs displaying normal asters in wild-type, dlkb1 and pins brains. Scale bar: 5 µm.