Fig. 9. dlkb1 and pins function in different pathways controlling the stability of spindle MTs in Drosophila. Mitotic figures from brains of dlkb1³¹⁵ pins^P62 double-mutants were stained for tubulin (Tub, green), DNA (blue) and Centrosomin (red). (A-F) NBs; (G-I) GMCs. (A,B) Metaphases; (C-E) anaphases; (F) telophase; (G) metaphases; (H) anaphase; (I) telophase. The arrow in E points to a lagging X chromosome with unseparated sister chromatids. Note the extremely defective spindle structures of the NBs shown in B,D,E,H. Scale bar: 5 µm. (J) Size distribution of metaphase spindles in wild-type and dlkb1 pins brains. Dpn-positive (NB) and Dpn-negative (GMC) spindles are depicted in red and green, respectively. Size (µm) classes: A, 4.7-6.9; B, 7.0-9.2; C, 9.3-11.5; D, 11.6-13.8; E, 13.9-16.1; F, 16.2-18.4; G, 18.5-20.7. (K) Expression of Pins and Dlkb1 in brains from third instar larvae of dlkb1, pins and dlkb1 pins mutants. Note that the Dlkb1 protein is undetectable in larval brain extracts of both dlkb1³¹⁵ homozygotes and dlkb1³¹⁵ pins^P62 double mutants. Similarly, Pins cannot be detected in brain extracts of both pins^P62/pins^P62 mutants and dlkb1³¹⁵ pins^P62 double mutants. The Giotto protein (Giansanti et al., 2006) was used as a loading control (LC).