Fig. 2. Predicted amino acid sequence of At-Delta cDNA in Achaearanea. (A) Domain organization of At-Delta protein. Two non-overlapping regions used to synthesize At-Delta^EB and At-Delta^HH dsRNAs are shown. (B) Alignment of the DSL domains of Delta and other proteins. Only residues not identical to those of At-Delta are displayed. (C) A neighbor-joining tree constructed using 156 amino acid sites in the DSL domain and EGF repeats 1-3. The numbers at the internal branches are bootstrap values (%). Accession numbers of proteins are as follows: At-Delta (AB287420), Cs-Delta1 (CAD45190), Cs-Delta2 (CAD45191), Dm-Delta (NP_477264), Sp-Delta (NP_001027542), Mm-Delta1 (NP_031891), Dr-DeltaD (NP_571030), Dm-Serrate (CAA40148), Mm-Jagged 1 (NP_038850). At, Achaearanea tepidariorum; Cs, Cupiennius salei; Dm, Drosophila melanogaster; Dr, Danio rerio; DSL, Delta/Serrate/LAG-2 domain; EGF, EGF repeat; Mm, Mus musculus; Sp, Strongylocentrotus purpuratus; SP, signal peptide; TM, transmembrane domain.