Fig. 5. Abnormal germ-band configurations following injection of At-Delta dsRNA in Achaearanea. (A-D) Flat preparations of a normal limb-extending embryo derived from #1/gfp (A) and embryos from #7/At-Delta^HH (B-D). All embryos in B-D were fixed at the same time. Prosomal regions of germ bands in A and B display similar shapes. Phenotypes of germ bands resulting from injection of At-Delta dsRNA are categorized as class 1 (B), class 2 (C) and class 3 (D). See text for details. (E) Relative numbers of embryos exhibiting normal, class 1, class 2 and class 3 phenotypes in the egg sacs indicated (see also Fig. 4). Data indicated by #9/At-Delta^HH were collected from an egg sac produced by another female 30 days after the first At-Delta^HH dsRNA injection. Scale bar: 100 µm.