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Figure 3


Fig. 3. Gonad microtubules are not required for proximal streaming in C. elegans. (A) Microtubules (red) and nuclei (blue) in pachytene region. Proximal is to the right. (B) High magnification image of microtubules around individual germ nuclei. (C) Microtubules extending between enlarging oocytes and gonad core. Oocyte membranes are indicated by dashed lines. (D) Summary diagram of gonad microtubules. (E,F) Gonads immunostained for {alpha}-tubulin after treating worms with empty-vector control (E) or an {alpha}-tubulin RNAi feeding strain (F) for 24 hours. (G,H) Normal particle movement in an {alpha}-tubulin RNAi-treated gonad; 2-minute particle tracks. Scale bars: 10 µm in A-C; 30 µm in E-G.