Fig. 4. The actomyosin cytoskeleton is required for streaming in C. elegans. (A-C) Phalloidin-stained microfilaments (green). (A) Low magnification view of enlarging oocytes in loop; fixation method I. (B) High magnification view of enlarging oocyte; fixation method II. Note the long filaments (arrowheads) extending from the core plasma membrane into the oocyte. (C) Pachytene region. (D) Summary diagram of gonad microfilaments. (E,F) Cytoplasmic streaming in gonad extruded in culture medium with EGTA; 2-minute particle tracks. (G,H) Abnormal, reversed streaming in gonad extruded in culture medium with latrunculin A and EGTA; 2-minute particle tracks. Scale bars: 10 µm in A-C; 30 µm in E,G.