Fig. 1. Grk requires Dpp to induce DV eggshell polarity. Anterior is to the left. (A-C) Double immunofluorescence stainings for Grk (green) and pMAD (red). (A,B) Lateral views. (C) Dorsal view. (D-F) Double immunofluorescence stainings for Grk (green) and ß-gal (red) of the egg chambers dissected from females carrying a dad-lacZ transgene. (D) Dorsal view. (E,F) Lateral views. (G,K) Immunofluorescence stainings for BR-C protein or Fas3 protein in wt stage 10B egg chambers; dorsal views. The white arrowheads mark the dorsal midline (DM). (H-J; L-N) Double immunofluorescence stainings for BR-C (red) or Fas3 (red) and GFP (green) in egg chambers carrying large Med¹³ mutant follicle cell clones; dorsal views. White lines mark the clone boundaries. White arrows in H mark single wt cells surrounded by cells mutant for Med¹³. (O) Immunofluorescence staining for ß-gal (red) of the egg chambers dissected from females carrying a pipe-lacZ transgene; lateral view. The white arrowhead marks the DM. (P-R) Double immunofluorescence stainings for ß-gal (red) to detect pipe-lacZ and GFP (green) in egg chambers carrying large Med¹³ mutant follicle cell clones; lateral views. White lines mark the clone boundaries. (S-U) Darkfield micrographs of deposited eggs. (S) Wild-type egg. (T) Egg laid by female mutant for cap-n-collar (cnc) lacks DV eggshell polarity. In cnc mutants the oocyte nucleus is not anchored at the dorsal-anterior cortex of the oocyte. This prevents the induction of DV polarity by the second round of Grk signaling (Guichet et al., 2001). (U) Egg laid by female in which follicle cell clones mutant for Med¹³ were induced also lacks DV eggshell polarity.