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Figure 3


Fig. 3. Combined misexpression of Dpp and Grk transforms all mainbody follicle cells into dorsal follicle cells. Anterior is to the left. (A-D) Lateral views of stage 10 egg chambers. (A-C) Double immunofluorescence staining for Grk (green) and pMAD (red). (D) Immunofluorescence stainings for pMAD. (A) Moderate misexpression of dpp (UASdpp x GR1-Gal4). (B) Strong misexpression of dpp (UASdpp x CY2-Gal4). (C) Strong misexpression of grk (UASpgrk x tub-Gal4VP16). (D) Simultaneous strong misexpression of grk (UASpgrk x tub-Gal4VP16) and dpp (UASdpp x CY2-Gal4). (Ea-Ja) Lateral view of darkfield micrographs of deposited eggs. (Eb-Jb) Immunofluorescence stainings for Fas3 (red) and DAPI (blue) of stage 10B egg chambers. Dorsal views, except in Gb, which shows a lateral view. (Ec-Jc) RNA in situ hybridization for BR-C on stage 10B egg chambers (dorsal view). (Ed-Jd) RNA in situ hybridization for kek on stage 10A egg chambers (lateral view) (Ea-d) Wild-type. (Fa-d) Moderate misexpression of dpp (UASdpp x GR1-Gal4). (Ga-d) Strong misexpression of dpp (UASdpp x CY2-Gal4). (Ha-d) Strong misexpression of grk (UASpgrk x tub-Gal4VP16). (Ia-d) Strong misexpression of grk and moderate misexpression of dpp. (Ja-d) Strong misexpression of both grk and dpp.