Fig. 4. Expression of dSno in ovaries and functional analysis of dSno and brk. Anterior is to the left. (A-C) RNA in situ hybridization for dSno. (A) Germarium and stage 1 to 4 egg chambers. (B) Lateral and (C) dorsal view of stage 10B egg chambers. (D-F) Double immunofluorescence for ß-gal (red) and BR-C (green). dSno-lacZ is expressed at both lateral and posterior boundaries of the BR-C domain at stage 10B. (G-I) Dorsal view of darkfield micrographs of deposited eggs. (J-L) Lateral view of darkfield micrographs of deposited eggs. White lines serve to measure the operculum size. (M-O) Lateral view of stage 14 egg chambers stained for DAPI (blue) and Fas3 (red). (G,J,M) Wild-type. (H,K,N) dSno¹⁷⁴/dSno¹⁷⁴. (I,L,O) Overexpression of dSno (UASdSno x CY2-Gal4). (P) Egg from female carrying large brk^M68 mutant follicle cell clones. The DAs are replaced by an enlarged operculum. (Q,R) Double immunofluorescence stainings for Fas3 (red) and GFP (green) in a stage 10B egg chamber carrying a large dorsally located brk^M68 mutant follicle cell clone.