Fig. 3. Time-lapse imaging of migrating neurons expressing either CAG-EGFP or CGC-Cdk5-DN. (A) Representative results are shown for mouse brain slices electroporated with either CAG-EGFP (GFP) or CGC-Cdk5-DN (Cdk5-DN) at E14.5. (B) Based on their morphology and behaviour, GFP-positive neurons were divided into two categories: bipolar shape (bipolar) or multipolar shape (multipolar) when observed at 4 hours (4 h) and 24 hours (24 h) post-electroporation, and the percentage of each is shown for CAG-EGFP and CGC-Cdk5-DN brain slices. (C) The percentage of GFP-positive neurons that transformed from multipolar to bipolar during a 20-hour peroid was calculated for each slice and subjected to statistical comparison. Multipolar-to-bipolar transformation was impaired in neurons expressing Cdk5-DN (GFP, 40.15±14.25%; Cdk5-DN, 7.75±3.77%, n=4); ^*, P<0.001.